Antibody Production Services

At Bio X Cell, we recognize the technical challenges and time demands of growing hybridoma cells and purifying antibodies in large quantities with low endotoxin levels. Our experienced team is here to manage this for you. Utilizing our optimized cell culture process, we produce antibodies more efficiently than traditional methods. A dedicated technical project manager will guide you through every step, offering recommendations and updates. Our Antibody Production Services are based in the U.S., ensuring streamlined communication, rapid turnaround time, and quality.

Project Workflow

Phase
1. Pilot Instructions for preparing and shipping cells.
Assessment of cell line activity utilizing a small scale cell culture followed by either protein A or G affinity purification.
Mycoplasma testing service.
Antibody isotyping service.
2. Cell Culture Cell culturing and monitoring 7 days/week.
Freezing of five vials of cells.
Storage of cells at Bio X Cell for rapid re-supply.
Expansion of cells using optimized cell culture process.
3. Purification Purification of supernatant on Protein A or G.
Buffer exchange to PBS that is preservative and stabilizer-free.
Sterile filtering of final product.
  • Testing to confirm <2EU/mg endotoxin.
  • <1EU/mg endotoxin level is available for an added charge.
4. Vialing and QC
  • Sterile vialing of finished product.
  • Custom aliquoting available.
Internal antibody quality assessment.
Certificate of Analysis.
Murine pathogen screening service.
5. Shipping Shipping on cold packs in environmentally friendly packaging.
james@2x

"When anyone asks me for CTLA-4 antibodies, I always refer them to you. I look forward to another 20 years."

Customer Testimonials