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How To Choose An Anti-PD-1 Antibody For Your Research

PD-1 Introduction

Programmed death-1 (PD-1) is an inhibitory receptor expressed on T-cells that promotes apoptosis of inflammatory T-cells and inhibits apoptosis of anti-inflammatory regulatory T-cells, thereby promoting self-tolerance and preventing autoimmune diseases. To evade immune surveillance, tumor cells often exploit this system by over-expressing PD-L1, the ligand which binds to and activates PD-1. Anti-PD-1 monoclonal antibodies are used to block the binding of PD-L1 to PD-1 which allows the immune system to find and kill tumor cells.

Anti-PD-1 Antibody Reactivity

The first step in deciding which anti-PD-1 antibody fits your research needs is to identify which species reactivity is needed for your experimental model. Bio X Cell offers anti-mouse and anti-human PD-1 antibodies. Which to choose is based on your experimental model. If you are using mice expressing mouse PD-1 you want to choose an anti-mouse PD-1 antibody. If you are using human cells in culture or humanized mice expressing human PD-1 then you want to choose an anti-human PD-1 antibody.

Anti-mouse PD-1 Antibody Clones

Detailed Comparison of anti-mouse PD-1 Antibody Clones

Clone RMP1-14 29F.1A12 J43
InVivoPlus™ Catalog no. BP0146 BP0273 BP0033-2
Isotype Rat IgG2a, κ Rat IgG2a Armenian Hamster IgG
First Citation In 2003 by Takanori Kanai et al In 2003 by Spencer C. Liang et al In 1996 by Yasutoshi Agata et al
Reported Applications in vivo blocking of PD-1/PD-L signaling
  • in vivo blocking of PD-1/PD-L signaling
  • in vitro PD-1 neutralization
  • Immunohistochemistry (frozen)
  • Immunofluorescence
  • Western blot
  • Flow cytometry
  • in vivo blocking of PD-1/PD-L signaling
  • in vitro PD-1 neutralization
  • Western blot
Immunogen Syrian Hamster BKH cells transfected with mouse PD-1 cDNA Recombinant PD-1-Ig fusion protein Syrian Hamster BKH cells transfected with mouse PD-1 cDNA
Purity >95%
Determined by SDS-PAGE
>95%
Determined by SDS-PAGE
>95%
Determined by SDS-PAGE
Stabilizers or Preservatives Free Free Free
Size 5, 25, 50, 100 mg and more 5, 25, 50, 100 mg and more 5, 25, 50, 100 mg and more
Availability In stock In stock In stock
Recommended Isotype Control InVivoPlus™ rat IgG2a isotype control, anti-trinitrophenol InVivoPlus™ rat IgG2a isotype control, anti-trinitrophenol InVivoPlus™ polyclonal Armenian hamster IgG
Recommended Dilution Buffer InVivoPure™ pH 7.0 Dilution Buffer InVivoPure™ pH 7.0 Dilution Buffer InVivoPure™ pH 6.5 Dilution Buffer

Recombinant anti-mouse PD-1 Antibody Clones

Bio X Cell also offers recombinant chimeric versions of our three anti-mouse PD-1 antibody clones. RecombiMAb™ antibodies are provided as chimeric antibodies with mouse constant regions instead of the typical rat or hamster constant regions. This ensures reduced immunogenicity in sensitive mouse models. Select RecombiMAb™ antibodies are also available in multiple Fc silenced versions. These Fc silenced versions have mutations in the Fc fragment of the antibody rendering them unable to bind to endogenous Fcγ receptors.

Detailed Comparison of Recombinant anti-mouse PD-1 Antibody Clones

Clone RMP1-14-CP153 RMP1-14-CP151 RMP1-14-CP162 29F.1A12™-CP159 RMP1-14-CP157 29F.1A12™-CP155
InVivoPlus™ Catalog no. CP153 CP151 CP162 CP159 CP157 CP155
Isotype Mouse IgG2a, κ Mouse IgG2a, κ Mouse IgG1, κ Mouse IgG2a, κ Mouse IgG2a, κ Mouse IgG2a, κ
Mutations LALA-PG D265A None None None LALA-PG
Reported Applications in vivo blocking of PD-1/PD-L signaling* *Reported for the original rat IgG2a RMP1-14 antibody
  • in vivo blocking of PD-1/PD-L signaling* *Reported for the original rat IgG2a RMP1-14 antibody
  • in vivo blocking of PD-1/PD-L signaling* *Reported for the original rat IgG2a RMP1-14 antibody
  • in vivo blocking of PD-1/PD-L signaling*
  • in vitro PD-1 neutralization*
  • Immunohistochemistry (frozen)*
  • Immunofluorescence*
  • Western blot*
  • Flow cytometry*
  • *Reported for the original rat IgG2a 29F.1A12 antibody
  • in vivo blocking of PD-1/PD-L signaling*
  • *Reported for the original rat IgG2a RMP1-14 antibody
  • in vivo blocking of PD-1/PD-L signaling*
  • in vitro PD-1 neutralization*
  • Immunohistochemistry (frozen)*
  • Immunofluorescence*
  • Western blot*
  • Flow cytometry*
  • *Reported for the original rat IgG2a 29F.1A12 antibody
Immunogen Syrian Hamster BKH cells transfected with mouse PD-1 cDNA Syrian Hamster BKH cells transfected with mouse PD-1 cDNA Syrian Hamster BKH cells transfected with mouse PD-1 cDNA Recombinant PD-1-Ig fusion protein Syrian Hamster BKH cells transfected with mouse PD-1 cDNA Recombinant PD-1-Ig fusion protein
Purity >95%
Determined by SDS-PAGE
>95%
Determined by SDS-PAGE
>95%
Determined by SDS-PAGE
>95%
Determined by SDS-PAGE
>95%
Determined by SDS-PAGE
>95%
Determined by SDS-PAGE
Stabilizers or Preservatives Free Free Free Free Free Free
Size 5, 25, 50, 100 mg and more 5, 25, 50, 100 mg and more 5, 25, 50, 100 mg and more 5, 25, 50, 100 mg and more 5, 25, 50, 100 mg and more 5, 25, 50, 100 mg and more
Availability In stock In stock In stock In stock In stock In stock
Recommended Isotype Control InVivoRecombiMAb mouse IgG2a (D265A) isotype control, anti-hen egg lysozyme InVivoRecombiMAb mouse IgG2a (D265A) isotype control, anti-hen egg lysozyme InVivoInVivoPlus mouse IgG1 isotype control, unknown specificity InVivoInVivoPlus mouse IgG2a isotype control, unknown specificity InVivoInVivoPlus mouse IgG2a isotype control, unknown specificity InVivoRecombiMAb mouse IgG2a (LALA-PG) isotype control, unknown specificity
Recommended Dilution Buffer InVivoPure™ pH 7.0 Dilution Buffer InVivoPure™ pH 7.0 Dilution Buffer InVivoPure™ pH 7.0 Dilution Buffer InVivoPure™ pH 7.0 Dilution Buffer InVivoPure™ pH 7.0 Dilution Buffer InVivoPure™ pH 7.0 Dilution Buffer

Anti-human PD-1 Antibody Clones

Bio X Cell also offers multiple anti-human PD-1 antibodies for use in humanized mouse models or immunoassays. These include clones J116 and J110 and Pembrolizumab and Nivolumab biosimilar antibodies.

Detailed Comparison of anti-human PD-1 Antibody Clones

Clone Nivolumab Pembrolizumab J116 407.6G12 J110
InVivoPlus™ Catalog no. SIM0003 SIM0010 BE0188 BE0387 BE0193
Isotype Human IgG4 Human IgG4 Mouse IgG1, κ Mouse IgG2a, κ Mouse IgG1, κ
Reported Applications
  • Blocking of PD-1/PD-L signaling
  • Western Blot
  • Immunohistochemistry
  • Flow cytometry
  • Blocking of PD-1/PD-L signaling
  • Functional assays
  • in vitro PD-1 neutralization
  • in vivo PD-1 blockade in humanized mice
  • Western Blot
  • Flow Cytometry
  • in vivo PD-1 blockade in humanized mice
  • Flow Cytometry
Immunogen Human PD-1 Human PD-1 Not available or unknown Human PD-1 p248Tyr peptide conjugated to KLH Not available or unknown
Purity >95%
Determined by SDS-PAGE
>95%
Determined by SDS-PAGE
>95%
Determined by SDS-PAGE
>95%
Determined by SDS-PAGE
>95%
Determined by SDS-PAGE
Stabilizers or Preservatives Free Free Free Free Free
Size 5, 25, 50, 100 mg and more 5, 25, 50, 100 mg and more 5, 25, 50, 100 mg and more 5, 25, 50, 100 mg and more 5, 25, 50, 100 mg and more
Availability In stock In stock In stock In stock In stock
Recommended Isotype Control InVivoRecombiMAb human IgG4 (S228P) isotype control, anti-hen egg lysozyme InVivoRecombiMAb human IgG4 (S228P) isotype control, anti-hen egg lysozyme InVivoInVivoMAb mouse IgG1 isotype control, unknown specificity InVivoInVivoMAb mouse IgG2a isotype control, unknown specificity InVivoInVivoMAb mouse IgG1 isotype control, unknown specificity
Recommended Dilution Buffer InVivoPure™ pH 7.0 Dilution Buffer InVivoPure™ pH 7.0 Dilution Buffer InVivoPure™ pH 7.0 Dilution Buffer InVivoPure™ pH 7.0 Dilution Buffer InVivoPure™ pH 7.0 Dilution Buffer